Initial Operation
Column temperature
Aurora Series columns are designed to operate optimally at 40–60 °C, with a maximum allowable temperature of 60 °C. For more information on column oven compatibility, click here.
Operating environmental conditions
The laboratory room temperature must be maintained between 15 and 27 °C (59 and 81 °F). The relative humidity of the operating environment must be between 20 and 80%, with no condensation. The operating environment of the equipment should be free of ...
Column volumes and equilibration
Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...
Sample loading
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...
Sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Important Loading contaminated samples onto the column may disrupt solvent flow ...
Spray voltage
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Important Exceeding a spray voltage of 2500V may result in damage ...
Storage of Aurora Series columns
Aurora Series columns should be stored in a cool, dry environment. Each column has a recommended shelf life of 24-months from the date of manufacture, making them easy to purchase in bulk and keep on hand for future use. Note To maintain optimal ...
Initial operation and buffer compositions
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 80% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...
Removal from a UHPLC
Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...
Standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...