Operation of Aurora Series columns - column temperature
The recommended operating temperature for Aurora Series columns is 40-60°C. The maximum operating temperature is 60°C.
For additional recommendations on operating conditions by column dimension, refer to
this section of the Help Centre.
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Operation of Aurora Series columns - column equilibration
Before each run the column should be reequilibrated using a minimum of 4 column volumes of 100% buffer A. Column equilibration should be performed below 1000 bar. Column volumes: Aurora Frontier, 60cm X 75μm: 2.65μl Aurora Ultimate, 25cm X 75μm: ...
Operation of Aurora Series columns - initial operation
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...
Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required. Removal of the nanoViper™ Fingertight ...
Operation of Aurora Series columns - standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
Operation of Aurora Series columns - sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Loading contaminated samples onto the column may disrupt solvent flow or foul ...