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Column volumes and equilibration
Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...
Rapid 8x75 column – Recommended conditions
Temperature & flow rate We have tested flow rates from 100 to 1000 nl/min and all produced great results. Maximum pressure The Aurora Series column and nanoZero® fitting can both handle pressures >1700 bar. Expected back pressure The below graph ...
Ultimate 25x75 column – Recommended conditions
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the Ultimate 25x75 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at ...
Ultimate 25x150 column – Recommended conditions
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the Ultimate 25x150 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at ...
Elite 15x150 column – Recommended conditions
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the Elite 15x150 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at optimal ...