Related Articles
Column volumes and equilibration
Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...
Demonstration of consistent results using the 5cm Aurora Rapid150 column
Tryptic digested Hela cell lysate was injected and separated using our 180 samples per day method and data dependent acquisition using PASEF to compare the peptide intensities across samples. This was achieved under the following conditions: Column: ...
25 cm Aurora Ultimate 25x150 column – Recommended conditions
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the Aurora Ultimate 25x150 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures ...
15 cm Aurora Elite 15x150 column – Recommended conditions
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the 15 cm Aurora Elite 15x150 column. Operation without a column oven may result in fluctuating operating temperatures and higher back ...
5 cm Aurora Rapid 5x75 column – Recommended conditions
Temperature & flow rate We tested flow rates ranging from 50 to 2000 nl/min on the 5 cm Aurora Rapid 5×75 column, all of which produced excellent results. At these flow rates and room temperature, the back pressure from the column should remain ...