Column volumes and equilibration

Column volumes and equilibration

Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A.

Column volumes:
  1. Frontier 60x75, 60 cm X 75μm: 2.65μl
  2. Ultimate 25x150, 25 cm X 150μm: 4.42μl
  3. Ultimate 25x75, 25 cm X 75μm: 1.1μl
  4. Elite 15x150, 15 cm X 150μm: 2.66μl
  5. Elite 15x75, 15 cm X 75μm: 0.66μl
  6. Rapid 8x150, 8 cm X 150μm: 1.41μl
  7. Rapid 8x75, 8 cm X 75um: 0.35μl
  8. Rapid 5x150, 5 cm X 150μm: 0.88μl
  9. Rapid 5x75, 5 cm X 75um: 0.22μl

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