Column volumes and equilibration
Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A.
Column volumes:
Aurora Frontier, 60 cm X 75μm: 2.65μl
Aurora Ultimate, 25 cm X 75μm: 1.1μl
Aurora Elite, 15 cm X 75μm: 0.66μl
Aurora 8 cm Rapid 8x150, 8 cm X 150μm: 1.41μl
Aurora 8 cm Rapid 8x75, 8 cm X 75um: 0.35μl
Aurora 5 cm Rapid 5x150, 5 cm X 150μm: 0.88μl
Aurora 5 cm Rapid 5x75, 5 cm X 75um: 0.22μl
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Initial operation and buffer compositions
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...
Sample loading
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...
Standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
Removal from a UHPLC
Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...
Column temperature
Aurora Series columns are designed to operate optimally at 40–60 °C, with a maximum allowable temperature of 60 °C. For more information on column oven compatibility, click here.