Operation of Aurora Series columns - column equilibration

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• Operation of Aurora Series columns - initial operation

  Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

• Operation of Aurora Series columns - sample loading & buffer compositions

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...

• Operation of Aurora Series columns - removal from a UHPLC

  If possible, do not remove the Aurora Series column from a UHPLC system. If removal is required, please follow the below instructions: Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back ...

• Operation of Aurora Series columns - standby and idle conditions

  To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...

• Operation of Aurora Series columns - sample preparation

  Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc).  We recommend performing the following on your sample: SP3, FASP or S-trap ...