Column volumes and equilibration

Column volumes and equilibration

For initial installation Aurora Series columns should be equilibrated via direct flow with 5 column volumes of 80% buffer B (100% Acetonitrile, 0.1% Formic Acid) followed by 5 column volumes of 1% buffer B. 

Warning
WARNING: Do not run UHPLC system column equilibration scripts during initial installation of IonOpticks columns. These scripts can cause irreversible column damage and will void the replacement policy. 
​
Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A.

Column volumes:
  1. Frontier 60x75, 60 cm X 75μm: 2.65μl
  2. Ultimate 25x150, 25 cm X 150μm: 4.42μl
  3. Ultimate 25x75, 25 cm X 75μm: 1.1μl
  4. Elite 15x150, 15 cm X 150μm: 2.66μl
  5. Elite 15x75, 15 cm X 75μm: 0.66μl
  6. Rapid 8x150, 8 cm X 150μm: 1.41μl
  7. Rapid 8x75, 8 cm X 75um: 0.35μl
  8. Rapid 5x150, 5 cm X 150μm: 0.88μl
  9. Rapid 5x75, 5 cm X 75um: 0.22μl

    • Related Articles

    • Initial operation and buffer compositions

      Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 80% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

    • Sample loading

      We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...

    • Example gradients for Aurora Series columns

    • Removal from a UHPLC

      To maintain optimal performance and extend column lifetime, we recommend keeping the column installed once in use. Avoid removing the column unless performance declines and replacement is required. If removal is necessary, please refer to the Removal ...

    • Standby and idle conditions

      To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...