Initial operation and buffer compositions

Initial operation and buffer compositions

Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable for several minutes. Voltage should be applied once the mobile phase reaches the emitter tip. It is recommended that at least one gradient is run without sample injection before assessment of column performance using standards.

We recommend the following buffer compositions:
  1. Buffer A: 99.9% MilliQ Water, 0.1% formic acid
  2. Buffer B: 99.9% Acetonitrile, 0.1% formic acid
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Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions.

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