Operation of Aurora Series columns - standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode:
- The instrument continues to run at the desired operating pressure
- The instrument ideally continues to run blank samples using mobile phase gradients typical of normal operation
- Spray voltages is maintained during these operations
Extended periods of time at isocratic flow will reduce column performance. Column performance can be recovered by running a blank gradient without sample injection.
Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the Aurora Series column from a UHPLC system. If removal is required, please follow the below instructions: Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back ...
Operation of Aurora Series columns - sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). We recommend performing the following on your sample: SP3, FASP or S-trap ...
Operation of Aurora Series columns - initial operation
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate of 400nl/min for around 10 minutes or until the pressure is stable for several minutes. Voltage ...
Operation of Aurora Series columns - sample loading & buffer compositions
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...
Operation of Aurora Series columns - column equilibration
Before each run the column should be reequilibrated using a minimum of 4 column volumes of 100% buffer A. Column equilibration should be performed below 1000 bar. Column volumes: Aurora Frontier, 60cm X 75μm: 2.65μl Aurora Ultimate, 25cm X 75μm: ...