Example gradients for Aurora Series columns

Example gradients for Aurora Series columns








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    • Initial operation and buffer compositions

      Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

    • Sample loading

      We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...

    • Demonstration of stable retention time with Aurora Series columns

      A HeLa tryptic digest sample was injected on 6 columns from independent production batches. The retention times for 15 selected peptides were analysed to demonstrate the intra- and inter-column retention time stability for Aurora Series columns. The ...

    • Column volumes and equilibration

      Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...

    • Expected phosphopeptide identifications using Aurora Series columns

      Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs.  These results were achieved under the following conditions: Column:  25cm Aurora Ultimate column Sample:  500ng ...