Example gradients for Aurora Series columns

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• Initial operation and buffer compositions

  Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

• Sample loading

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...

• Demonstration of stable retention time with Aurora Series columns

  A HeLa tryptic digest sample was injected on 6 columns from independent production batches. The retention times for 15 selected peptides were analysed to demonstrate the intra- and inter-column retention time stability for Aurora Series columns. The ...

• Column volumes and equilibration

  Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...

• 8 cm Aurora Rapid 8x150 column – Recommended conditions

  Temperature & flow rate We tested flow rates ranging from 1 to 4 μL/min on the 8 cm Aurora Rapid 8×150 column, all of which produced excellent results. At these flow rates and room temperature, the back pressure from the column should remain within ...