Example gradients for Aurora Series columns

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• Initial operation and buffer compositions

  Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 80% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

• Column volumes and equilibration

  For initial installation Aurora Series columns should be equilibrated via direct flow with 5 column volumes of 80% buffer B (100% Acetonitrile, 0.1% Formic Acid) followed by 5 column volumes of 1% buffer B. WARNING: Do not run UHPLC system column ...

• Sample loading

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...

• Demonstration of stable retention time with Aurora Series columns

  A HeLa tryptic digest sample was injected on 6 columns from independent production batches. The retention times for 15 selected peptides were analysed to demonstrate the intra- and inter-column retention time stability for Aurora Series columns. The ...

• Expected phosphopeptide identifications using Aurora Series columns

  Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs.  These results were achieved under the following conditions: Column:  25cm Aurora Ultimate column Sample:  500ng ...