Sample loading

Sample loading

We recommend the following buffer compositions:
  1. Buffer A: 99.9% MilliQ Water, 0.1% formic acid
  2. Buffer B: 99.9% Acetonitrile, 0.1% formic acid
Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions


The maximum load capacity that each Aurora Series column can withstand is outlined below:
  1. Frontier 60x75: 1μg per hour of gradient
  2. Ultimate 25x150: 3μg
  3. Ultimate 25x75: 1μg
  4. Elite 15x150: 2μg
  5. Elite 15x75: 500ng
  6. Rapid 8x150: 500ng
  7. Rapid 8x75: 100ng
  8. Rapid 5x150: 200ng
  9. Rapid 5x75: 50ng
The optimal column load for your experiments depends on the specific MS instrument you are using. Please consult your MS guidelines to determine the ideal load for your experiments. 

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