Operation of Aurora Series columns - sample loading & buffer compositions

Operation of Aurora Series columns - sample loading & buffer compositions

We recommend the following buffer compositions:
  1. Buffer A: 99.9% MilliQ Water, 0.1% formic acid
  2. Buffer B: 99.9% Acetonitrile, 0.1% formic acid
Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions.

The optimal column load for your experiments depends on the specific MS instrument you are using. Please consult your MS guidelines to determine the ideal load for your experiments. 

The maximum load that each Aurora Series column can withstand is outlined below:
  1. 60cm Aurora Frontier: 1μg per hour of gradient
  2. 25cm Aurora Ultimate: 1μg
  3. 15cm Aurora Elite: 500ng
  4. 8cm Rapid8x150: 500ng
  5. 8cm Rapid8x75: 100ng
  6. 5cm Rapid5x150: 200ng
  7. 5cm Rapid5x75: 50ng



    • Related Articles

    • Operation of Aurora Series columns - initial operation

      Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

    • Operation of Aurora Series columns - sample preparation

      Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Loading contaminated samples onto the column may disrupt solvent flow or foul ...

    • Operation of Aurora Series columns - column equilibration

      Before each run the column should be re-equilibrated using a minimum of 4 column volumes of 100% buffer A. Column volumes: Aurora Frontier, 60cm X 75μm: 2.65μl Aurora Ultimate, 25cm X 75μm: 1.1μl Aurora Elite, 15cm X 75μm: 0.66μl Aurora 8cm Rapid ...

    • Operation of Aurora Series columns - standby and idle conditions

      To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...

    • Operation of Aurora Series columns - removal from a UHPLC

      If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required. Removal of the nanoViper™ Fingertight ...