Sample loading

Sample loading

We recommend the following buffer compositions:
  1. Buffer A: 99.9% MilliQ Water, 0.1% formic acid
  2. Buffer B: 99.9% Acetonitrile, 0.1% formic acid
Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions


The maximum load capacity that each Aurora Series column can withstand is outlined below:
  1. 60 cm Aurora Frontier: 1μg per hour of gradient
  2. 25 cm Aurora Ultimate: 1μg
  3. 15 cm Aurora Elite: 500ng
  4. 8 cm Rapid8x150: 500ng
  5. 8 cm Rapid8x75: 100ng
  6. 5 cm Rapid5x150: 200ng
  7. 5 cm Rapid5x75: 50ng
The optimal column load for your experiments depends on the specific MS instrument you are using. Please consult your MS guidelines to determine the ideal load for your experiments. 

    • Related Articles

    • Initial operation and buffer compositions

      Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

    • Sample preparation

      Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Important Loading contaminated samples onto the column may disrupt solvent flow ...

    • Column volumes and equilibration

      Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Aurora Frontier, 60 cm X 75μm: 2.65μl Aurora Ultimate, 25 cm X 75μm: 1.1μl Aurora Elite, 15 cm X 75μm: 0.66μl Aurora 8 ...

    • Standby and idle conditions

      To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...

    • Removal from a UHPLC

      Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...