Operation of Aurora Series Columns - Sample Loading & Buffer Compositions

Operation of Aurora Series Columns - Sample Loading & Buffer Compositions

We recommend the following buffer compositions:
  1. Buffer A: 99.9% MilliQ Water, 0.1% formic acid
  2. Buffer B: 99.9% Acetonitrile, 0.1% formic acid
Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions.

The optimal column load depends on the MS instrument that is being used:
  1. For Bruker timsTOF Pro and timsTOF Flex, 200-400ng will generally produce the most identifications.
  2. For Thermo Orbitrap instruments, a 500ng-1μg load will produce good results.
It should be noted that an increase in carryover between samples may be observed when higher amounts of peptides are loaded which can require blanks to be run between samples to clean up the column.



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