Operation of Aurora Series columns - sample loading & buffer compositions
We recommend the following buffer compositions:
Buffer A: 99.9% MilliQ Water, 0.1% formic acid
Buffer B: 99.9% Acetonitrile, 0.1% formic acid
Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions.
The optimal column load for your experiments depends on the specific MS instrument you are using. Please consult your MS guidelines to determine the ideal load for your experiments.
The maximum load that each Aurora Series column can withstand is outlined below:
Aurora Frontier 60 cm x 75 μm: | 1μg per hour of gradient |
Aurora Ultimate 25cm x 75 μm: | 1μg |
Aurora Elite 15 cm x 75 μm: | 500ng |
| Aurora Rapid 8 cm x 150 μm: | 500ng |
| Aurora Rapid 8 cm x 75 μm: | 100ng |
Aurora Rapid 5 cm x 150 μm: | 200ng |
Aurora Rapid 5 cm x 75 μm: | 50ng |
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