Storage of Aurora Series columns

Aurora Series columns should be stored in a cool, dry environment. Each column has a recommended shelf life of 24-months from the date of manufacture, making them easy to purchase in bulk and keep on hand for future use.

Note
To maintain optimal performance and extend column lifetime, we recommend keeping the column installed once in use. If removal is required, please refer to our Removal from a UHPLC article for proper handling instructions.

• Related Articles

• Column volumes and equilibration

  Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Aurora 60 cm Frontier, 60 cm X 75μm: 2.65μl Aurora 25 cm Ultimate 25x150, 25 cm X 150μm: 4.42μl Aurora 25 cm Ultimate ...

• Column temperature

  Aurora Series columns are designed to operate optimally at 40–60 °C, with a maximum allowable temperature of 60 °C. For more information on column oven compatibility, click here.

• Removal from a UHPLC

  Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...

• Sample preparation

  Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Important Loading contaminated samples onto the column may disrupt solvent flow ...

• Sample loading

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...