Factors that cause ionisation issues with Aurora Series columns
Spray instability can occasionally occur at high %ACN concentrations. This can normally be mitigated by ensuring that the nanoViper™ Fingertight Fitting is installed as instructed in the user guide.
If
you only observe spray sputtering for a small period of time in each
gradient then it should not significantly affect the results of an
experiment.
There are a number of additional factors that can cause ionisation issues with your Aurora Series column. The most common causes are:
A slightly fouled emitter tip from an impure sample. This can clear with subsequent injections if the amount of material deposited on the emitter is small.
Degassing of buffers. We recommend degassing and refreshing buffers weekly.
Obstructive particles collecting in the nanoZero® union causing cavitation.
An under tight nanoViper™/nanoZero® union. Tighten the nanoViper™ Fitting Finger tight until you reach a firm stop. Operation of the column with an under tight
fitting can result in a small leak and spray instability that can not be recovered with additional tightening.