Demonstration of stable retention time with Aurora Series columns

Demonstration of stable retention time with Aurora Series columns

A HeLa tryptic digest sample was injected on 6 columns from independent production batches. The retention times for 15 selected peptides were analysed to demonstrate the intra- and inter-column retention time stability for Aurora Series columns. The 3 points for each column represent run number 2, 5 and 10. Numbers in orange correspond to the peptide description in Table 1. This was achieved under the following conditions:

Column: 
25cm Aurora Ultimate column
Sample: 
100ng HeLa tryptic digest
Gradient: 
5% to 35% B 90min gradient
Flow Rate: 
400nl/min
Temperature: 
50°C
MS: 
Bruker Impact II

This demonstrates that retention times remain consistent across all peptides and Aurora Series columns. 



                    


Tryptic digested Hela cell lysate was injected and separated using our 180 samples per day method and data dependent acquisition using PASEF to compare the retention time across samples. This was achieved under the following conditions:

Column: 
5cm Aurora Rapid150 column
Sample: 
80ng Hela cell tryptic digest
Gradient: 
5% to 34% B 5min gradient
Flow Rate: 
2ul/min
Temperature: 
Room temperature
MS: 
Bruker timsTOF Pro

This demonstrates that our 5cm Aurora Rapid150 column achieves highly reproducible peptide retention times.