Expected phosphopeptide identifications using Aurora Series columns
Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs.
These results were achieved under the following conditions:
25cm Aurora Ultimate column
500ng phospho-peptide (IMAC) enriched human acute monocytic leukaemia cell tryptic digests
Thermo Q-Exactive HF-X
This demonstrates a high number of phosphopeptide identifications across multiple runs.