Expected phosphopeptide identifications using Aurora Series columns
Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs.
These results were achieved under the following conditions:
Column: | 25cm Aurora Ultimate column |
Sample: | 500ng phospho-peptide (IMAC) enriched human acute monocytic leukaemia cell tryptic digests |
Gradient: | 120min |
Flow Rate: | 400nl/min |
MS: | Thermo Q-Exactive HF-X |
This demonstrates a high number of phosphopeptide identifications across multiple runs.
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