Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the Aurora Series column from a UHPLC system. If removal is required, please follow the below instructions:
- Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back pressure has stabilised below 10 bar
- Set MS system into standby mode
The nanoViper™ Fingertight Fitting can then be removed from the nanoZero®.
For removal of the column for extended periods of time we recommend placing 30 μl of methanol in the nanoZero® and screwing in the plug supplied during transport.
Removal of the nanoViper™ Fingertight Fitting from the nanoZero® under high back pressure conditions can damage the stationary phase bed and lead to blockages and poor chromatographic performance.
The removal procedure can lead to fouling of the emitter tip and poor column performance. The IonOpticks replacement guarantee is not valid if a column has been removed from a UHPLC once in operation.
Operation of Aurora Series columns - standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
Operation of Aurora Series columns - initial operation
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate of 400nl/min for around 10 minutes or until the pressure is stable for several minutes. Voltage ...
Operation of Aurora Series columns - sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). We recommend performing the following on your sample: SP3, FASP or S-trap ...
Operation of Aurora Series columns - sample loading & buffer compositions
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...
Operation of Aurora Series columns - column equilibration
Before each run the column should be reequilibrated using a minimum of 4 column volumes of 100% buffer A. Column equilibration should be performed below 1000 bar. Column volumes: 25cm X 75μm: 1.1μl 15cm X 75μm: 0.66μl 5cm X 150μm: 0.88μl