Operation of Aurora Series Columns - Removal from a UHPLC
If possible, do not remove the Aurora Series column from a UHPLC system. If removal is required, please follow the below instructions:
- Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back pressure has stabilised below 10 bar.
- Set MS system into standby mode.
- The nanoViper™ Fingertight Fitting can now be removed from the nanoZero®.
For removal of the column for extended periods of time we recommend placing 30 μl of methanol in the nanoZero® and screwing in the plug supplied during transport.
Removal of the nanoViper™ Fingertight Fitting from the nanoZero® under high back pressure conditions can damage the stationary phase bed and lead to blockages and poor chromatographic performance.
The removal procedure can lead to fouling of the emitter tip and poor column performance. The IonOpticks replacement guarantee is not valid if a column has been removed from a UHPLC once in operation.
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