Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required.
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Removal of the nanoViper™ Fingertight Fitting from the nanoZero® under high back pressure conditions can damage the stationary phase bed and lead to blockages and poor chromatographic performance.
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The removal procedure can lead to fouling of the emitter tip and poor column performance. The IonOpticks replacement guarantee is not valid if a column has been removed from a UHPLC once in operation.
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For removal of the column for extended periods of time we recommend placing 30 μl of methanol in the nanoZero® and screwing in the plug supplied during transport.
Removal of Aurora and Aurora CSI columns:
- Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back pressure has stabilised below 10 bar
- Set MS system into standby mode
- The nanoViper™ Fingertight Fitting can then be removed from the nanoZero®.
- Screw the shipping plug into the nanoZero®. For removal of the column for extended periods of time we recommend placing 30μl of methanol in the nanoZero® before installing the shipping plug
Removal of Aurora TS columns from Thermo Scientific's Nanospray Flex source:
- Run 80% B for 5min at operating flow rates
- Reduce flow to 0.002 μl/min for 10 min or until the back pressure has stabilised below 10 bar
- Set MS system into standby mode
- Slide Nanospray Flex stage out of the safety hood before sliding the column cassette backwards from the TS Nanospray Flex Adapter along the column holder
- The nanoViper™ Fingertight Fitting can now be removed from the nanoZero®
- Carefully place the protective cap back onto the cassette and press firmly to ensure that the cap is held securely
- Screw the shipping plug into the nanoZero®. For removal of the column for extended periods of time we recommend placing 30μl of methanol in the nanoZero® before installing the shipping plug
Removal of Aurora TS columns from Thermo Scientific's EasySpray source:
- Run 80% B for 5min at operating flow rates
- Reduce flow to 0.002 μl/min for 10 min or until the back pressure has stabilised below 10 bar
- Set MS system into standby mode
- Slide column cassette backwards from EasySpray source along the column holder
- The nanoViper™ Fingertight Fitting can now be removed from the nanoZero®
- Carefully place the protective cap back onto the cassette and press firmly to ensure that the cap is held securely
- Screw the shipping plug into the nanoZero®. For removal of the column for extended periods of time we recommend placing 30μl of methanol in the nanoZero® before installing the shipping plug
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