Operation of Aurora Series columns - removal from a UHPLC

If possible, do not remove the Aurora Series column from a UHPLC system. If removal is required, please follow the below instructions:

1. Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back pressure has stabilised below 10 bar
   
2. Set MS system into standby mode

The nanoViper™ Fingertight Fitting can then be removed from the nanoZero®.

For removal of the column for extended periods of time we recommend placing 30 μl of methanol in the nanoZero® and screwing in the plug supplied during transport.

Removal of the nanoViper™ Fingertight Fitting from the nanoZero® under high back pressure conditions can damage the stationary phase bed and lead to blockages and poor chromatographic performance.

The removal procedure can lead to fouling of the emitter tip and poor column performance. The IonOpticks replacement guarantee is not valid if a column has been removed from a UHPLC once in operation.

• Related Articles

• Operation of Aurora Series columns - standby and idle conditions

  To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...

• Operation of Aurora Series columns - initial operation

  Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

• Operation of Aurora Series columns - sample preparation

  Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc).  We recommend performing the following on your sample: SP3, FASP or S-trap ...

• Operation of Aurora Series columns - column equilibration

  Before each run the column should be reequilibrated using a minimum of 4 column volumes of 100% buffer A. Column equilibration should be performed below 1000 bar. Column volumes: Aurora Frontier, 60cm X 75μm: 2.65μl Aurora Ultimate, 25cm X 75μm: ...

• Operation of Aurora Series columns - sample loading & buffer compositions

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...