Operation of Aurora Series Columns - Sample Preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc).
We recommend performing the following on your sample:
SP3, FASP or S-trap assisted digestion
De-salt with C18 Stage-tips
Loading contaminated samples onto the column may disrupt solvent flow or foul the emitter tip leading to a loss of performance.
Please submit a support ticket if you would like further details on recommended sample preparation protocols.
Operation of Aurora Series Columns - Standby and Idle Conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
Operation of Aurora Series Columns - Initial Operation
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate of 400nl/min for around 10 minutes or until the pressure is stable for several minutes. Voltage ...
Operation of Aurora Series Columns - Sample Loading & Buffer Compositions
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...
Operation of Aurora Series Columns - Removal from a UHPLC
If possible, do not remove the Aurora Series column from a UHPLC system. If removal is required, please follow the below instructions: Run 80% B for 5min at operating flow rates before reducing flow to 0.002 μl/min for 10min or until the back ...
Operation of Aurora Series Columns - Column Equilibration
Before each run the column should be reequilibrated using a minimum of 4 column volumes of 100% buffer A. Column equilibration should be performed below 1000 bar. Column volumes: 25cm X 75μm: 1.1μl 15cm X 75μm: 0.66μl 5cm X 150μm: 0.88μl