Sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc).
Important
Loading contaminated samples onto the column may disrupt solvent flow or foul the emitter tip leading to a loss of performance.
Loading contaminated samples onto the column may disrupt solvent flow or foul the emitter tip leading to a loss of performance.
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Initial operation and buffer compositions
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...Standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...Sample loading
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...Removal from a UHPLC
Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...Emitter tip replacements
The stationary phase in our column is packed directly into the emitter tip to eliminate post-column dead volume. Therefore, the emitter cannot be replaced as a separate item. Provided that samples loaded on to the column are free of salts and other ...