Sample preparation

Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). 

Important 
Loading contaminated samples onto the column may disrupt solvent flow or foul the emitter tip leading to a loss of performance. 

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• Initial operation and buffer compositions

  Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

• Standby and idle conditions

  To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...

• Sample loading

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...

• Removal from a UHPLC

  Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...

• Column volumes and equilibration

  Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Aurora 60 cm Frontier, 60 cm X 75μm: 2.65μl Aurora 25 cm Ultimate 25x150, 25 cm X 150μm: 4.42μl Aurora 25 cm Ultimate ...