Operation of Aurora Series columns - spray voltage
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Exceeding a spray voltage of 2500V may result in damage to the emitter.
Operation of Aurora Series columns - initial operation
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...
Operation of Aurora Series columns - standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required. Removal of the nanoViper™ Fingertight ...
Operation of Aurora Series columns - sample loading & buffer compositions
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...
Operation of Aurora Series columns - column temperature
The recommended operating temperature for Aurora Series columns is 40-60°C. The maximum operating temperature is 60°C. For additional recommendations on operating conditions by column dimension, refer to this section of the Help Centre.