Spray voltage

It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V.

Important
Exceeding a spray voltage of 2500V may result in damage to the emitter.

• Related Articles

• Standby and idle conditions

  To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...

• Sample loading

  We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...

• Initial operation and buffer compositions

  Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...

• Removal from a UHPLC

  Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...

• Column temperature

  Aurora Series columns are designed to operate optimally at 40–60 °C, with a maximum allowable temperature of 60 °C. For more information on column oven compatibility, click here.