Spray voltage

Spray voltage

It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V.

AlertImportant
Exceeding a spray voltage of 2500V may result in damage to the emitter.

    • Related Articles

    • Factors that cause ionisation issues with Aurora Series columns

      Spray instability can occasionally occur at high %ACN concentrations. This can normally be mitigated by ensuring that the nanoViper™ Fingertight Fitting is installed as instructed in the user guide. If you only observe spray sputtering for a small ...
    • Standby and idle conditions

      To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
    • How to reduce high column back pressure caused by a collection of particles that has transferred from the sample vial to the start of the column

      This is the most common cause for increased column back pressure. To reduce this column back pressure we recommend raising the heater temperature to a maximum of 60°C.
    • Sample loading

      We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...
    • Initial operation and buffer compositions

      Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 80% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...