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Factors that cause ionisation issues with Aurora Series columns
Spray instability can occasionally occur at high %ACN concentrations. This can normally be mitigated by ensuring that the nanoViper™ Fingertight Fitting is installed as instructed in the user guide. If you only observe spray sputtering for a small ...
Standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
How to reduce high column back pressure caused by a collection of particles that has transferred from the sample vial to the start of the column
This is the most common cause for increased column back pressure. To reduce this column back pressure we recommend raising the heater temperature to a maximum of 60°C.
Sample loading
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...
Initial operation and buffer compositions
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 80% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...