The stationary phase used in our columns has a lower retentivity of peptides than other stationary phases (peptides are eluted at a lower %ACN than other stationary phases). Therefore, there is a small amount of peptides that will not bind to the ...
Our columns are subjected to rigorous quality control procedures under the direct eye of our Senior Scientists. All IonOpticks products are covered by our 100% performance guarantee. Any item not meeting our high-performance expectations due to ...
The presence of small concentrations of HEPES should not affect the column chemistry, however, there is a significant risk of fouling the emitter tip leading to spray instability as HEPES is not volatile and may deposit on the tip.
A moderate increase in back pressure is expected over the lifetime of the column. When this gradual increase of back pressure is experienced, it generally does not affect performance. This can be reduced, although not eliminated, by using digest ...
A thick sample is often the result of precipitate forming after the addition of loading buffer to a sample vial. There are two things you can try to revive the column: Run 5 - 10+ blank gradients Run 85% acetonitrile at 400nl/min for an extended ...
This is the most common cause for increased column back pressure. To reduce this column back pressure we recommend raising the heater temperature to a maximum of 60°C.
Spray instability can occasionally occur at high %ACN concentrations. This can normally be mitigated by ensuring that the nanoViper™ Fingertight Fitting is installed as instructed in the user guide. If you only observe spray sputtering for a small ...
The stationary phase in our column is packed directly into the emitter tip to eliminate post-column dead volume. Therefore, the emitter cannot be replaced as a separate item. Provided that samples loaded on to the column are free of salts and other ...
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Important Exceeding a spray voltage of 2500V may result in damage ...
This video demonstrates how to connect the nanoZero to the Thermo Scientific nanoViper™ fitting: Hold the nanoZero with the spanner. Tighten the nanoViper™ fitting finger tight until you reach a firm stop. A firm stop is the point at which the ...
Aurora Series columns are available in four specific compatibility marks, allowing for compatibility with Thermo Scientific, Bruker, and Sciex instruments: Instrument Compatibility Aurora Columns Compatible for use with Thermo Scientific’s Nanospray ...
Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...