How to revive a column that has been blocked by a 'thick' sample

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• Managing a gradual increase in back pressure over a column's lifetime

  A moderate increase in back pressure is expected over the lifetime of the column. When this gradual increase of back pressure is experienced, it generally does not affect performance. This can be reduced, although not eliminated, by using digest ...

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• Peptides from a sample that are not captured during the loading step

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