Low amounts (1mM - 10mM) of HEPES in a sample

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• Peptides from a sample that are not captured during the loading step

  The stationary phase used in our columns has a lower retentivity of peptides than other stationary phases (peptides are eluted at a lower %ACN than other stationary phases). Therefore, there is a small amount of peptides that will not bind to the ...

• How to revive a column that has been blocked by a 'thick' sample

  A thick sample is often the result of precipitate forming after the addition of loading buffer to a sample vial. There are two things you can try to revive the column: Run 5 - 10+ blank gradients Run 85% acetonitrile at 400nl/min for an extended ...

• How to reduce high column back pressure caused by a collection of particles that has transferred from the sample vial to the start of the column

  This is the most common cause for increased column back pressure. To reduce this column back pressure we recommend raising the heater temperature to a maximum of 60°C.

• Factors that cause ionisation issues with Aurora Series columns

  Spray instability can occasionally occur at high %ACN concentrations. This can normally be mitigated by ensuring that the nanoViper™ Fingertight Fitting is installed as instructed in the user guide. If you only observe spray sputtering for a small ...

• Emitter tip replacements

  The stationary phase in our column is packed directly into the emitter tip to eliminate post-column dead volume. Therefore, the emitter cannot be replaced as a separate item. Provided that samples loaded on to the column are free of salts and other ...