Low amounts (1mM - 10mM) of HEPES in a sample
The presence of small concentrations of HEPES should not affect the column chemistry, however, there is a significant risk of fouling the emitter tip leading to spray instability as HEPES is not volatile and may deposit on the tip.
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Sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Important Loading contaminated samples onto the column may disrupt solvent flow ...
Peptides from a sample that are not captured during the loading step
The stationary phase used in our columns has a lower retentivity of peptides than other stationary phases (peptides are eluted at a lower %ACN than other stationary phases). Therefore, there is a small amount of peptides that will not bind to the ...
Standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
Performance of Aurora Series columns with phosphoproteomic samples
Our columns perform very well with phosphoproteomic samples and we have a number of customers which have successfully used our columns for phosphopeptide samples. Below are a few examples of phosphoproteomic publications that have used our columns: ...
Sample loading
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...