How to reduce high column back pressure caused by a collection of particles that has transferred from the sample vial to the start of the column
This is the most common cause for increased column back pressure. To reduce this column back pressure we recommend raising the heater temperature to a maximum of 60°C.
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Managing a gradual increase in back pressure over a column's lifetime
A moderate increase in back pressure is expected over the lifetime of the column. When this gradual increase of back pressure is experienced, it generally does not affect performance. This can be reduced, although not eliminated, by using digest ...
Sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Important Loading contaminated samples onto the column may disrupt solvent flow ...
Removal from a UHPLC
Where possible, we recommend not to remove the column from a UHPLC system before performance falls and the column requires replacement. However, removal may sometimes be required for instrument servicing or maintenance. For more information on ...
How to revive a column that has been blocked by a 'thick' sample
A thick sample is often the result of precipitate forming after the addition of loading buffer to a sample vial. There are two things you can try to revive the column: Run 5 - 10+ blank gradients Run 85% acetonitrile at 400nl/min for an extended ...
Standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...