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What if there is a fault with my column?
Our columns are subjected to rigorous quality control procedures under the direct eye of our Senior Scientists. All IonOpticks products are covered by our 100% performance guarantee. Any item not meeting our high-performance expectations due to ...
Sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Important Loading contaminated samples onto the column may disrupt solvent flow ...
Performance comparison between Aurora Series columns and competitors
The data in the bar graph below was achieved under the following conditions: Competitor 1 column: 50cm X 75μm Commercial solution without integrated tip Competitor 2 column: 50cm X 75μm Commercial solution with integrated emitter tip IonOpticks ...
Low amounts (1mM - 10mM) of HEPES in a sample
The presence of small concentrations of HEPES should not affect the column chemistry, however, there is a significant risk of fouling the emitter tip leading to spray instability as HEPES is not volatile and may deposit on the tip.
Initial operation and buffer compositions
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 80% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...