NanoShield® Traps
Installing & Removing the NanoShield® Reverse-Flush Trap on your UHPLC
This article demonstrates how to install the NanoShield™ Reverse-Flush Trap on your UHPLC. The NanoShield™ 5 cm × 100 µm trap is designed for on-valve use with reverse-flush (backward) operation only. Using it in forward-flush mode may compromise ...
NanoShield® Trap Column | User Guide
Access a copy of the User Manual: NanoShield® Trap Column here.
Operation of NanoShield® Trap
Once the trap column is connected to your UHPLC system, begin operation using 80-85% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes, after which time the pressure should be stable. It is recommended that at ...
NanoShield® Application Note
Traditionally, some level of peptide loss has been considered an acceptable trade-off when using trap columns. NanoShield™, however, delivers near-direct injection performance. Testing showed less than a 1% reduction in protein identifications and ...
NanoShield® Trap: Frequently Asked Questions (FAQs)
What is the NanoShield® Trap? Samples often contain salts, detergents, and other particulates that can interfere with mass spectrometry performance. A trap column is used as an inline chromatographic (clean-up) step to retain analytes of interest ...
Can I use Aurora Series columns with a trapping column?
Aurora Series columns may be used in a one column mode (direct injection) or paired with a trapping column. The NanoShield™ C18 traps from IonOpticks are designed to maximise peptide retention and proteome coverage. Unlike traditional trap columns, ...