NanoShield Traps
Operation of NanoShield™ Trap
Once the trap column is connected to your UHPLC system, begin operation using 85% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes, after which time the pressure should be stable. It is recommended that at ...
Installing & Removing the NanoShield™ Reverse-Flush Trap on your UHPLC
This article demonstrates how to install the NanoShield™ Reverse-Flush Trap on your UHPLC. The NanoShield™ 5 cm × 100 µm trap is designed for on-valve use with reverse-flush (backward) operation only. Using it in forward-flush mode may compromise ...
NanoShield™ Application Note
Traditionally, some level of peptide loss has been considered an acceptable trade-off when using trap columns. NanoShield™, however, delivers near-direct injection performance. Testing showed less than a 1% reduction in protein identifications and ...
NanoShield™ Trap: Frequently Asked Questions (FAQs)
What is the NanoShield™ Trap? Samples often contain salts, detergents, and other particulates that can interfere with mass spectrometry performance. A trap column is used as an inline chromatographic (clean-up) step to retain analytes of interest ...