Installing & Removing the NanoShield® Reverse-Flush Trap on your UHPLC
Installing & Removing the NanoShield® Reverse-Flush Trap on your UHPLC
This article demonstrates how to install the NanoShield™ Reverse-Flush Trap on your UHPLC. The NanoShield™ 5 cm × 100 µm trap is designed for on-valve use with reverse-flush (backward) operation only. Using it in forward-flush mode may compromise performance.
For optimal fitting, installation requires two short capillaries (such as Thermo Scientific™ nanoViper 30 µm ID × 10 cm) and a 1/4 inch spanner.
Aurora Series columns may be used in a one column mode (direct injection) or paired with a trapping column. The NanoShield™ C18 traps from IonOpticks are designed to maximise peptide retention and proteome coverage. Unlike traditional trap columns, ...
Once the trap column is connected to your UHPLC system, begin operation using 80-85% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes, after which time the pressure should be stable. It is recommended that at ...
What is the NanoShield® Trap? Samples often contain salts, detergents, and other particulates that can interfere with mass spectrometry performance. A trap column is used as an inline chromatographic (clean-up) step to retain analytes of interest ...
This guide walks you through setting up, fitting, and installing your Aurora Series columns with your UHPLC system. Setup of UHPLC Systems Aurora Series columns may be used in either: One-column mode (direct injection) With a trapping column If using ...