Setup of UHPLC Systems

Setup of UHPLC Systems

This guide walks you through setting up, fitting, and installing your Aurora Series columns with your UHPLC system.

Setup of UHPLC Systems
Aurora Series columns may be used in either:
  1. One-column mode (direct injection)
  2. With a trapping column

If using Aurora Series column in direct injection mode:
Please refer to manufacturer's guidelines for operating your UHPLC in a one column mode.

If using Aurora Series column with a trapping column:
We have tested a range of commercially available trap columns for use with our Aurora Series columns and have identified the PepMap™ Neo 5 µm C18 300 µm X 5 mm Trap Cartridge as the most compatible. We still get great results and only lose ~5-8% of protein IDs with the inclusion of a trap. In order to achieve the best results, it is absolutely necessary to load and equilibrate the column with 0% ACN. Please keep this in mind if you wish to use the PepMap column with an Aurora column.

Warning
Safety Note:
Handling fused-silica or glass tubing and tips can cause serious personal injury. Always wear:
  • Safety goggles (meeting AS/NZS 1336 standards or equivalent).
  • Puncture- and chemical-resistant gloves should be worn at all times.

Fitting the Column to Your UHPLC System





Installing the Column
For more information on installing your column, visit the Installation Guide section of our Help Centre, where you’ll find instructional videos and step-by-step guidance tailored to your ion source.