Setup of UHPLC Systems

This guide walks you through setting up, fitting, and installing your Aurora Series columns with your UHPLC system.

Setup of UHPLC Systems

Aurora Series columns may be used in either:

1. One-column mode (direct injection)
   
2. With a trapping column
   
   

If using Aurora Series column in direct injection mode:

Please refer to manufacturer's guidelines for operating your UHPLC in a one column mode.

If using Aurora Series column with a trapping column:

NanoShield™ C18 traps are designed to maximise peptide retention and proteome coverage. Unlike traditional trap columns, NanoShield™ retains hydrophilic peptides, such as, early-eluting post-translationally modified peptides (e.g., phosphopeptides and glycopeptides) that are often lost during sample loading.  Testing showed less than a 1% reduction in protein identifications and only a 1–3% reduction in precursor identifications compared with direct injection, when evaluated against a comparable trap column. For more information, please refer to the NanoShield™ Application Note.

Safety Note:
Handling fused-silica or glass tubing and tips can cause serious personal injury. Always wear:

• Safety goggles (meeting AS/NZS 1336 standards or equivalent).
• Puncture- and chemical-resistant gloves should be worn at all times.
  

Fitting the Column to Your UHPLC System

Installing the Column

For more information on installing your column, visit the Installation Guide section of our Help Centre, where you’ll find instructional videos and step-by-step guidance tailored to your ion source.

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