NanoShield™ Application Note

NanoShield™ Application Note

Traditionally, some level of peptide loss has been considered an acceptable trade-off when using trap columns. NanoShield™, however, delivers near-direct injection performance. Testing showed less than a 1% reduction in protein identifications and only a 1–3% reduction in precursor identifications compared with direct injection, when evaluated against a comparable trap column.

For further details, you can refer to the NanoShield™ Application Note.


    • Related Articles

    • NanoShield™ Trap: Frequently Asked Questions (FAQs)

      What is the NanoShield™ Trap? Samples often contain salts, detergents, and other particulates that can interfere with mass spectrometry performance. A trap column is used as an inline chromatographic (clean-up) step to retain analytes of interest ...

    • Setup of UHPLC Systems

      This guide walks you through setting up, fitting, and installing your Aurora Series columns with your UHPLC system. Setup of UHPLC Systems Aurora Series columns may be used in either: One-column mode (direct injection) With a trapping column If using ...

    • Operation of NanoShield™ Trap

      Once the trap column is connected to your UHPLC system, begin operation using 85% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes, after which time the pressure should be stable. It is recommended that at ...

    • Installing & Removing the NanoShield™ Reverse-Flush Trap on your UHPLC

      This article demonstrates how to install the NanoShield™ Reverse-Flush Trap on your UHPLC. The NanoShield™ 5 cm × 100 µm trap is designed for on-valve use with reverse-flush (backward) operation only. Using it in forward-flush mode may compromise ...