Choosing the ideal Aurora column length for your workflows

Choosing the ideal Aurora column length for your workflows

Key Considerations for Column Selection 

Choosing the ideal Aurora Series column length depends on several key factors, including your sample type, cohort size, throughput demands, and specific analytical performance goals.

Aurora Series columns are available in five length formats—60 cm Frontier, 25 cm Ultimate, 15 cm Elite, 8 cm Rapid, and 5 cm Rapid—each engineered to balance coverage, sensitivity, and throughput for different experimental needs. All columns 25 cm and shorter are also available in both nanoflow (75 µm) and capillary flow (150 µm) inner diameters, offering greater flexibility to suit different flow rates and sensitivity requirements.

This guide summarises the use cases, performance metrics, and technical specifications for each Aurora column format to help you make an informed decision. For more detailed comparisons of coverage, throughput, and sensitivity, please visit the product pages in our shop.


Aurora Frontier 60x75
(60cm x 75µm ID, 1.7µm C18)

Achieve near-complete proteome coverage with longer gradients and maximum depth.
  1. Sample throughput: 5–15 samples/day
  2. Flow rate: 100–300 nL/min
  3. Recommended gradients: 90–180 minutes
  4. Available in: Aurora, Aurora CSI, Aurora XT


Aurora Ultimate 25x75
(25cm x 75µm ID, 1.7µm C18)

Laboratory workhorse for a broad range of applications.
  • Sample throughput: 10–40 samples/day
  • Flow rate: 100-400 nL/min
  • Recommended gradients: 45–120 minutes
  • Available in: Aurora, Aurora CSI, Aurora XT, Aurora XS


Aurora Ultimate 25x150
(25cm x 150µm ID, 1.7µm C18)

Laboratory workhorse for a broad range of applications.
  • Sample throughput: 10–50 samples/day
  • Flow rate: 0.4-1.5 µL/min
  • Recommended gradients: 30–120 minutes
  • Available in: Aurora, Aurora CSI, Aurora XT, Aurora XS


Aurora Elite 15x75
(15cm x 75µm ID, 1.7µm C18)

Perfect balance between sensitivity and throughput for mid-sized cohorts.
  • Sample throughput: 30-60 samples/day
  • Flow rate: 100-600 nL/min
  • Recommended gradients: 10–45 minutes
  • Available in: Aurora, Aurora CSI, Aurora XT, Aurora XS


Aurora Elite 15x150
(15cm x 150µm ID, 1.7µm C18)

Perfect balance between sensitivity and throughput for mid-sized cohorts.
  • Sample throughput: 30-100 samples/day
  • Flow rate: 0.4-2.5 µL/min
  • Recommended gradients: 10–45 minutes
  • Available in: Aurora, Aurora CSI, Aurora XT, Aurora XS


Aurora Rapid 8x75 
(8cm x 75µm ID, 1.7µm C18)

Designed for fast, large-cohort analysis with good coverage.
  1. Sample throughput: 50–100 samples/day
  2. Flow rate: 100–1000 nL/min
  3. Recommended gradients: 10–30 minutes
  4. Available in: Aurora CSI, Aurora XT


Aurora Rapid 8x150
(8cm x 150µm ID, 1.7µm C18)

Designed for fast, large-cohort analysis with good coverage.
  1. Sample throughput:  50-100 samples/day
  2. Flow rate: 0.4-3 µL/min
  3. Recommended gradients: 5–15 minutes
  4. Available in: Aurora CSI, Aurora XT


Aurora Rapid 5x75
(5cm x 75µm ID, 1.7µm C18)

High-throughput and sensitivity for single-cell workflows.
  • Sample throughput: 80-300 samples/day
  • Flow rate: 100-2,000 nL/min
  • Recommended gradients: 5–15 minutes
  • Available in: Aurora, Aurora CSI, Aurora XT


Aurora Rapid 5x150
(5cm x 150µm ID, 1.7µm C18)

High-throughput analysis of large sample cohorts.
  • Sample throughput: 80-300 samples/day
  • Flow rate: 0.4-4 µL/min
  • Recommended gradients: 5–15 minutes
  • Available in: Aurora, Aurora CSI, Aurora XT

InfoNote: Column lifetime estimates are based on recommended gradient conditions. Actual performance may vary depending on your instrument setup, operating pressure, sample preparation quality, and overall system maintenance.

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