Recommended conditions for 60cm Aurora Frontier column
Temperature & flow rate
A column oven must be installed at the mass spectrometer source for the 60cm Aurora Frontier column. For typical operation we recommend a flow rate between 200 and 300 nl/min with the column oven set to 60°C. The maximum operating temperature is 60°C. Flow rates can be increased or decreased to suit particular applications.
Maximum pressure
Aurora Series columns can withstand pressures >1700 bar (Gen3) and >1200bar (Gen2).
Example gradient
Refer to
this Help Centre article for an example gradient.
Expected back pressure
Refer to
this Help Centre article for the expected backpressure of a 60cm Aurora Frontier column, for a given flow rate at 60°C.
For additional details on the operation of Aurora Series columns, please refer to
this Help Centre section.
Related Articles
Recommended conditions for 15cm Aurora Elite column
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the 15cm Aurora Elite column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at ...
Recommended conditions for 25cm Aurora Ultimate column
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the 25cm Aurora Ultimate column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at ...
Recommended conditions for 5cm Aurora Rapid75 column
Temperature & flow rate We have tested flow rates from 50 to 2000 nl/min and all produced great results. At flow rates of between 50 and 2000 nl/min at room temperature, the back pressure from the column should be within normal operating range of ...
Recommended conditions for 5cm Aurora Rapid150 column
Temperature & flow rate We have tested flow rates from 2 to 4 μl/min and all produced great results. At flow rates of between 2 and 4 μl/min at room temperature, the back pressure from the column should be within normal operating range of most ...
Expected back pressure, 60cm Aurora Frontier column
The below graph depicts the expected backpressure of a 60cm Aurora Frontier column, for a given flow rate at 60°C. This graph was recorded after 50 sample injections. The buffer composition is 79.9% water, 20% acetonitrile, 0.1% formic acid. The ...