Recommended conditions for 5cm Aurora Rapid75 column

Recommended conditions for 5cm Aurora Rapid75 column

Temperature & flow rate
We have tested flow rates from 50 to 2000 nl/min and all produced great results. At flow rates of between 50 and 2000 nl/min at room temperature, the back pressure from the column should be within normal operating range of most UHPLCs. Higher flow rates generally require heating to keep the back pressure within manageable levels.

Maximum pressure
Aurora Series columns can withstand pressures >1700 bar (Gen3) and >1200bar (Gen2).

Example gradient
Refer to this Help Centre article for an example gradient.

Expected back pressure
Refer to this Help Centre article for the expected backpressure of a 5cm Aurora Rapid75 column, for a given flow rate at both room temperature and 40°C.


For additional details on the operation of Aurora Series columns, please refer to this Help Centre section.

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