Recommended conditions for 25cm Aurora Ultimate column

Recommended conditions for 25cm Aurora Ultimate column

Temperature & flow rate
We recommend the use of a column oven installed at the mass spectrometer source for the 25cm Aurora Ultimate column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at optimal flow rates, generating inconsistent sample separation.

For typical operation we recommend a flow rate around 400 nl/min with the column oven set to 40-60°C. This will ensure UHPLC back pressures of around 600-700 bar. The maximum operating temperature is 60°C. Flow rates can be increased or decreased to suit particular applications.

Maximum pressure
Aurora Series columns can withstand pressures >1700 bar (Gen3) and >1200bar (Gen2).

Example gradient
Refer to this Help Centre article for an example gradient.

Expected back pressure
Refer to this Help Centre article for the expected backpressure of a 25cm Aurora Ultimate column, for a given flow rate at both room temperature and 40°C.


For additional details on the operation of Aurora Series columns, please refer to this Help Centre section.

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