15 cm Aurora Elite 15x75 column – Recommended conditions
Temperature & flow rate
We recommend the use of a column oven installed at the mass spectrometer source for the 15 cm Aurora Elite 15x75 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at optimal flow rates, generating inconsistent sample separation.
For typical operation recommend a flow rate around 400 nl/min with the column oven set to 40-60°C. This will ensure UHPLC back pressures of around 400-500 bar. The maximum operating temperature is 60°C. Flow rates can be increased or decreased to suit particular applications.
Maximum pressure
Aurora Series columns and nanoZero® fitting can both handle pressures can withstand pressures >1700 bar.
The below graph depicts the expected backpressure of a 15 cm Aurora Elite column, for a given flow rate at both room temperature and 40°C. The buffer composition is 79.9% water, 20% acetonitrile, 0.1% formic acid.
Example gradient
See example gradients for the full range of Aurora Series columns here.
Related Articles
15 cm Aurora Elite 15x150 column – Recommended conditions
Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the 15 cm Aurora Elite 15x150 column. Operation without a column oven may result in fluctuating operating temperatures and higher back ...
60 cm Aurora Frontier 60x75 column – Recommended conditions
Temperature & flow rate A column oven must be installed at the mass spectrometer source for the 60 cm Aurora Frontier column. For typical operation we recommend a flow rate between 200 and 300 nl/min with the column oven set to 60°C. The maximum ...
5 cm Aurora Rapid 5x75 column – Recommended conditions
Temperature & flow rate We tested flow rates ranging from 50 to 2000 nl/min on the 5 cm Aurora Rapid 5×75 column, all of which produced excellent results. At these flow rates and room temperature, the back pressure from the column should remain ...
5 cm Aurora Rapid 5x150 column – Recommended conditions
Temperature & flow rate We tested flow rates ranging from 1 to 4 μL/min on the 5 cm Aurora Rapid 5×150 column, all of which produced excellent results. At these flow rates and room temperature, the back pressure from the column should remain within ...
8 cm Aurora Rapid 8x150 column – Recommended conditions
Temperature & flow rate We tested flow rates ranging from 1 to 4 μL/min on the 8 cm Aurora Rapid 8×150 column, all of which produced excellent results. At these flow rates and room temperature, the back pressure from the column should remain within ...