Operation
Operation of Aurora Series columns - column temperature
The recommended operating temperature for Aurora Series columns is 40-60°C. The maximum operating temperature is 60°C. For additional recommendations on operating conditions by column dimension, refer to this section of the Help Centre.
Operation of Aurora Series columns - environmental conditions
The laboratory room temperature must be maintained between 15 and 27 °C (59 and 81 °F). The relative humidity of the operating environment must be between 20 and 80%, with no condensation. The operating environment of the equipment should be free of ...
Operation of Aurora Series columns - column equilibration
Before each run the column should be re-equilibrated using a minimum of 4 column volumes of 100% buffer A. Column volumes: Aurora Frontier, 60cm X 75μm: 2.65μl Aurora Ultimate, 25cm X 75μm: 1.1μl Aurora Elite, 15cm X 75μm: 0.66μl Aurora 8cm Rapid ...
Operation of Aurora Series columns - sample loading & buffer compositions
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these ...
Operation of Aurora Series columns - sample preparation
Prior to loading samples onto your Aurora Series column, the sample should be de-salted and should not contain any contaminants (salts, detergents, solid particles, etc). Loading contaminated samples onto the column may disrupt solvent flow or foul ...
Operation of Aurora Series columns - spray voltage
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Exceeding a spray voltage of 2500V may result in damage to the ...
Operation of Aurora Series columns - initial operation
Once the column is connected to your UHPLC system and is placed inside the source heater or housing, begin operation using 70% buffer B at a flow rate equivalent to the desired gradient flow rate for around 10 minutes or until the pressure is stable ...
Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required. Removal of the nanoViper™ Fingertight ...
Operation of Aurora Series columns - standby and idle conditions
To optimise column lifetime and performance, we recommend that the following conditions are met while your instrument is standby and idle mode: The instrument continues to run at the desired operating pressure The instrument ideally continues to run ...
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Aurora Series column features
Aurora Series columns are available in four product ranges, allowing for compatibility with Thermo Scientific, Bruker, and Sciex instruments: Aurora columns Compatible with Thermo Scientific's Nanospray Flex ion source. Aurora XT columns Compatible ...
Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required. Removal of the nanoViper™ Fingertight ...
Operation of Aurora Series columns - spray voltage
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Exceeding a spray voltage of 2500V may result in damage to the ...
Aurora Series column and UHPLC compatibility
Aurora Series columns are compatible with a wide range of LC-MS systems. Compatibility is not limited to equipment presented below. Please submit a support ticket if you have any questions regarding your instrument compatibility. Bruker nanoElute ...
Difference between Gen2 and Gen3 Aurora Series columns
Delivering unrivalled coverage, throughput, sensitivity and reproducibility, Gen3 Aurora Series columns embody all of the strengths of previous generations, augmented by three years of intense research, development and industry collaboration. ...