Expected performance of 5cm Aurora Rapid75 column
A Hela tryptic digest (1 ng) was separated on a 5cm Aurora Rapid75 column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nl/min. The extracted ion chromatograms for 7 selected peptides from the analysis are shown. The average Full Width at Half Maximum (FWHM, seconds) for each peptide across 30 runs is shown above each peak.
The retention times of the 7 peptides analysed in the adjacent figure were plotted across 30 runs to demonstrate retention time stability for the 5cm Aurora Rapid75 column.
A dilution series of a HeLa tryptic digest was separated on a 5 Aurora Rapid75 column using an Evosep One and Bruker timsTOF SCP (dia-PASEF) using an 80 samples per day method, n = 6. Data courtesy of Dr Christoph Krisp (Bruker), Dorte Bekker-Jensen and Dr Nicolai Bache (Evosep).