Expected performance of 5cm Aurora Rapid 5x75 column
A Hela tryptic digest (1 ng) was separated on a 5cm Aurora Rapid 5x75 column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nl/min. The extracted ion chromatograms for 7 selected peptides from the analysis are shown. The average Full Width at Half Maximum (FWHM, seconds) for each peptide across 30 runs is shown above each peak.
The retention times of the 7 peptides analysed in the adjacent figure were plotted across 30 runs to demonstrate retention time stability for the 5cm Aurora Rapid 5x75 column.
A dilution series of a HeLa tryptic digest was separated on a 5 Aurora Rapid 5x75 column using an Evosep One and Bruker timsTOF SCP (dia-PASEF) using an 80 samples per day method, n = 6. Data courtesy of Dr Christoph Krisp (Bruker), Dorte Bekker-Jensen and Dr Nicolai Bache (Evosep).
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Column volumes and equilibration
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The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 200ng Hela tryptic digest on a 25cm Aurora Ultimate column using different gradient lengths. Samples were run on a Waters M-class UPLC ...Expected performance of 15cm Aurora Elite column
The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 80ng Hela tryptic digest on a 15cm Aurora Elite column using different gradient lengths. Samples were run on a Waters M-class UPLC ...Expected phosphopeptide identifications using Aurora Series columns
Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs. These results were achieved under the following conditions: Column: 25cm Aurora Ultimate column Sample: 500ng ...Rapid 8x75 column – Recommended conditions
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