Expected performance of 5cm Aurora Rapid 5x150 column
Tryptic digested Human Plasma was injected and separated using our 180 samples per day method and data dependent acquisition using PASEF resulting in >230 protein identifications (>1,600 unique peptide identifications) [Fig1, Fig 2] with highly reproducible peptide retention times between runs [Fig 3].
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Expected performance of 5cm Aurora Rapid 5x75 column
A Hela tryptic digest (1 ng) was separated on a 5cm Aurora Rapid 5x75 column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nl/min. The extracted ion chromatograms for 7 selected peptides from the ...
Expected performance of 25cm Aurora Ultimate column
The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 200ng Hela tryptic digest on a 25cm Aurora Ultimate column using different gradient lengths. Samples were run on a Waters M-class UPLC ...
Expected performance of 15cm Aurora Elite column
The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 80ng Hela tryptic digest on a 15cm Aurora Elite column using different gradient lengths. Samples were run on a Waters M-class UPLC ...
Column volumes and equilibration
Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...
Expected phosphopeptide identifications using Aurora Series columns
Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs. These results were achieved under the following conditions: Column: 25cm Aurora Ultimate column Sample: 500ng ...