Expected performance of 25cm Aurora Ultimate column

Expected performance of 25cm Aurora Ultimate column

The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 200ng Hela tryptic digest on a 25cm Aurora Ultimate column using different gradient lengths. Samples were run on a Waters M-class UPLC coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN.






Yeast tryptic digest was spiked into a Hela tryptic digest (200ng) in different ratios:

Sample A: 45ng
Sample B: 15ng

Samples were run on a Waters M-class UPLC coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN. Each point represents a unique protein with ratios calculated between the A and B samples. The boxplot demonstrates the average ratio and interquartile range of data. Expected ratios are indicated with dashed lines.


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