Expected performance of 25cm Aurora Ultimate column
The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 200ng Hela tryptic digest on a 25cm Aurora Ultimate column using different gradient lengths. Samples were run on a Waters M-class UPLC coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN.
Yeast tryptic digest was spiked into a Hela tryptic digest (200ng) in different ratios:
Sample A: 45ng
Sample B: 15ng
Samples were run on a Waters M-class UPLC coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN. Each point represents a unique protein with ratios calculated between the A and B samples. The boxplot demonstrates the average ratio and interquartile range of data. Expected ratios are indicated with dashed lines.
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