Expected performance of 15cm Aurora Elite column

Expected performance of 15cm Aurora Elite column

The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 80ng Hela tryptic digest on a 15cm Aurora Elite column using different gradient lengths. Samples were run on a Waters M-class UPLC coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN.




Yeast tryptic digest was spiked into a Hela tryptic digest 200ng in different ratios:

Sample A: 45ng
Sample B: 15ng

Samples were run on a Waters M-class UPLC coupled to a Bruker timsTOF Pro, dia-PASEF acquisition. Data analysed using DIA-NN. Each point represents a unique protein with ratios calculated between the A and B samples. The boxplot demonstrates the average ratio and interquartile range of data. Expected ratios are indicated with dashed lines.



    • Related Articles

    • Expected performance of 25cm Aurora Ultimate column

      The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 200ng Hela tryptic digest on a 25cm Aurora Ultimate column using different gradient lengths. Samples were run on a Waters M-class UPLC ...

    • Elite 15x150 column – Recommended conditions

      Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the Elite 15x150 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at optimal ...

    • Elite 15x75 column – Recommended conditions

      Temperature & flow rate We recommend the use of a column oven installed at the mass spectrometer source for the Elite 15x75 column. Operation without a column oven may result in fluctuating operating temperatures and higher back pressures at optimal ...

    • Column volumes and equilibration

      Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...

    • Expected performance of 5cm Aurora Rapid 5x75 column

      A Hela tryptic digest (1 ng) was separated on a 5cm Aurora Rapid 5x75 column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nl/min. The extracted ion chromatograms for 7 selected peptides from the ...