Expected peak widths using the 5cm Aurora Rapid 5x150 column

The below boxplot demonstrates FWHM (seconds) from tryptic digested Human Plasma using our 180 samples per day method. The white line indicates median time.

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  The below boxplot demonstrates FWHM (seconds) from phospho-peptide (TiO2) enriched samples across 70 injections using a 25cm Aurora Ultimate column. This demonstrates consistent peak widths across multiple samples using Aurora Series columns. Tryptic ...

• Expected peak widths using the 15cm Aurora Elite column

  The below plot demonstrates FWHM (seconds) from 80ng Hela cell tryptic digest using different gradients lengths.

• Expected peak widths using the 25cm Aurora Ultimate column

  The below plot demonstrates FWHM (seconds) from 200ng Hela cell tryptic digest using different gradients lengths.

• Expected performance of 5cm Aurora Rapid 5x75 column

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• Column volumes and equilibration

  Before each run, Aurora Series columns should be equilibrated with a minimum of 4 column volumes of 100% buffer A. Column volumes: Frontier 60x75, 60 cm X 75μm: 2.65μl Ultimate 25x150, 25 cm X 150μm: 4.42μl Ultimate 25x75, 25 cm X 75μm: 1.1μl Elite ...