Application Notes And Performance Examples | Performance Examples

Performance Examples
Expected performance of 5cm Aurora Rapid 5x75 column
A Hela tryptic digest (1 ng) was separated on a 5cm Aurora Rapid 5x75 column using a Thermo Scientific Vanquish Neo UHPLC and TSQ Quantis across a 15 min sample gradient at 100 nl/min. The extracted ion chromatograms for 7 selected peptides from the ...
Expected performance of 5cm Aurora Rapid 5x150 column
Tryptic digested Human Plasma was injected and separated using our 180 samples per day method and data dependent acquisition using PASEF resulting in >230 protein identifications (>1,600 unique peptide identifications) [Fig1, Fig 2] with highly ...
Expected performance of 15cm Aurora Elite column
The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 80ng Hela tryptic digest on a 15cm Aurora Elite column using different gradient lengths. Samples were run on a Waters M-class UPLC ...
Expected performance of 25cm Aurora Ultimate column
The below graphs represent identified unique proteins, peptides, and average Full Width at Half Maximum (FWHM) from 200ng Hela tryptic digest on a 25cm Aurora Ultimate column using different gradient lengths. Samples were run on a Waters M-class UPLC ...
Expected peak widths using the 25cm Aurora Ultimate column
The below plot demonstrates FWHM (seconds) from 200ng Hela cell tryptic digest using different gradients lengths.
Expected peak widths using the 5cm Aurora Rapid 5x150 column
The below boxplot demonstrates FWHM (seconds) from tryptic digested Human Plasma using our 180 samples per day method. The white line indicates median time.
Expected peak widths using the 15cm Aurora Elite column
The below plot demonstrates FWHM (seconds) from 80ng Hela cell tryptic digest using different gradients lengths.
Demonstration of consistent results over multiple experiments and hundreds of injections
80 ng of HeLa peptide was injected and separated on a single 15cm Aurora Elite column across eight proteomics experiments. Sample types include: Cell Lysates Immunoprecipitated Samples Plasma Samples Urine Samples Cell Surface Capture This ...
Demonstration of consistent results using the 5cm Aurora Rapid150 column
Tryptic digested Hela cell lysate was injected and separated using our 180 samples per day method and data dependent acquisition using PASEF to compare the peptide intensities across samples. This was achieved under the following conditions: Column: ...
Performance of Aurora Series columns with phosphoproteomic samples
Our columns perform very well with phosphoproteomic samples and we have a number of customers which have successfully used our columns for phosphopeptide samples. Below are a few examples of phosphoproteomic publications that have used our columns: ...
Demonstration of consistent peak widths across multiple samples using Aurora Series columns
The below boxplot demonstrates FWHM (seconds) from phospho-peptide (TiO2) enriched samples across 70 injections using a 25cm Aurora Ultimate column. This demonstrates consistent peak widths across multiple samples using Aurora Series columns. Tryptic ...
Expected phosphopeptide identifications using Aurora Series columns
Counts shown on the Y-axis below represent the number of unique phosphorylated peptides identified across 4 replicate runs. These results were achieved under the following conditions: Column: 25cm Aurora Ultimate column Sample: 500ng ...
Performance comparison between Aurora Series columns and competitors
The data in the bar graph below was achieved under the following conditions: Competitor 1 column: 50cm X 75μm Commercial solution without integrated tip Competitor 2 column: 50cm X 75μm Commercial solution with integrated emitter tip IonOpticks ...
BPC: Typical results for Hela Tryptic Digest for 25cm Ultimate, 15cm Elite, and 5cm Rapid Aurora Series columns
The below base peak chromatogram represents typical results of a Hela cell tryptic digest with a 25cm Aurora Series column. This was achieved under the following conditions: Column: 25cm Aurora Ultimate column Sample: 200ng Hela cell tryptic digest ...
Demonstration of stable retention time with Aurora Series columns
A HeLa tryptic digest sample was injected on 6 columns from independent production batches. The retention times for 15 selected peptides were analysed to demonstrate the intra- and inter-column retention time stability for Aurora Series columns. The ...

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Aurora Series column features
Aurora Series columns are available in four product ranges, allowing for compatibility with Thermo Scientific, Bruker, and Sciex instruments: Aurora columns Compatible with Thermo Scientific's Nanospray Flex ion source. Aurora XT columns Compatible ...
Operation of Aurora Series columns - removal from a UHPLC
If possible, do not remove the column from a UHPLC system before the column performance falls and the columns requires replacement, however, removal for instrument servicing and maintenance is sometimes required. Removal of the nanoViper™ Fingertight ...
Operation of Aurora Series columns - spray voltage
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Exceeding a spray voltage of 2500V may result in damage to the ...
Aurora Series column and UHPLC compatibility
Aurora Series columns are compatible with a wide range of LC-MS systems. Compatibility is not limited to equipment presented below. Please submit a support ticket if you have any questions regarding your instrument compatibility. Bruker nanoElute ...
Difference between Gen2 and Gen3 Aurora Series columns
Delivering unrivalled coverage, throughput, sensitivity and reproducibility, Gen3 Aurora Series columns embody all of the strengths of previous generations, augmented by three years of intense research, development and industry collaboration. ...

Application Notes And Performance Examples | Performance Examples | Help Centre

Performance Examples

Expected performance of 5cm Aurora Rapid 5x75 column

Expected performance of 5cm Aurora Rapid 5x150 column

Expected performance of 15cm Aurora Elite column

Expected performance of 25cm Aurora Ultimate column

Expected peak widths using the 25cm Aurora Ultimate column

Expected peak widths using the 5cm Aurora Rapid 5x150 column

Expected peak widths using the 15cm Aurora Elite column

Demonstration of consistent results over multiple experiments and hundreds of injections

Demonstration of consistent results using the 5cm Aurora Rapid150 column

Performance of Aurora Series columns with phosphoproteomic samples

Demonstration of consistent peak widths across multiple samples using Aurora Series columns

Expected phosphopeptide identifications using Aurora Series columns

Performance comparison between Aurora Series columns and competitors

BPC: Typical results for Hela Tryptic Digest for 25cm Ultimate, 15cm Elite, and 5cm Rapid Aurora Series columns

Demonstration of stable retention time with Aurora Series columns

Popular Articles

Aurora Series column features

Operation of Aurora Series columns - removal from a UHPLC

Operation of Aurora Series columns - spray voltage

Aurora Series column and UHPLC compatibility

Difference between Gen2 and Gen3 Aurora Series columns