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Sample loading
We recommend the following buffer compositions: Buffer A: 99.9% MilliQ Water, 0.1% formic acid Buffer B: 99.9% Acetonitrile, 0.1% formic acid Please consult with your UHPLC manufacturer to confirm that your UHPLC is compatible with these compositions ...
Understanding your capillary fitting: what to look for before you connect
One of the most overlooked factors in maintaining consistent, high-quality spray in nano-scale liquid chromatography is something you handle every time you connect your column: your capillary fitting (Thermo Scientific nanoViper Fingertight Fittings ...
Removal from a UHPLC
To maintain optimal performance and extend column lifetime, we recommend keeping the column installed once in use. Avoid removing the column unless performance declines and replacement is required. If removal is necessary, please refer to the Removal ...
Spray voltage
It is recommended to start with a spray voltage of 1500V and increase over time as required to maintain a stable spray. It is recommended that the spray voltage does not exceed 2500V. Important Exceeding a spray voltage of 2500V may result in damage ...
Example gradients for Aurora Series columns